Clubs · 2000
13 clubs
| Club | League | Based in | Home ground |
|---|---|---|---|
| Boston Breakers | Women's United Soccer Association | Boston | Nickerson Field |
| Colorado Rapids U-23 | USL League Two | — | Dick's Sporting Goods Park |
| Colorado Springs Ascent | USL League Two | — | Switchbacks Training Stadium |
| Dallas Roma F.C. | North Texas Premier Soccer Association | Dallas | — |
| Dayton Gemini | USL League Two | — | Baujan Field |
| F.C. Indiana | Women's Premier Soccer League Elite | — | — |
| Lafayette Swamp Cats | USL League Two | — | — |
| Philadelphia Charge | Women's United Soccer Association | — | Villanova Stadium |
| Tampa Bay Hawks | USL League Two | — | — |
| Utah Blitzz | USL Second Division | Salt Lake City | Rice–Eccles Stadium |