| AC Crusaders | — | 2010 | Egg Harbor Township High School |
| Alabama Spirit | — | 2007 | — |
| Albuquerque Asylum | — | 2004 | Menaul School |
| Asheville City SC | Asheville | 2016 | Memorial Stadium, Asheville |
| Atlanta Silverbacks Reserves | Atlanta | 2007 | Atlanta Silverbacks Park |
| Atlantic City Diablos | — | 2007 | St. Augustine College Preparatory School |
| Bay Area Ambassadors | — | 2009 | Chabot College |
| Boston Aztec | — | 2006 | — |
| Brooklyn Italians | New York City | 1949 | John Dewey High School |
| Buffalo City FC | — | 2008 | Nichols School |
| Cape Coral Hurricanes | — | 2010 | Cape Coral High School |
| CD Aguiluchos USA | Oakland | 2012 | — |
| Charlottesville Alliance FC | Charlottesville | 2016 | Charlottesville High School |
| Charm City FC | — | 2007 | Arundel High School |
| Chesterfield United FC | — | 2013 | Williams Stadium |
| Chicago Fire NPSL | — | 2007 | SeatGeek Stadium |
| Chico Rooks | Chico | 1993 | University Soccer Stadium, Chico |
| Cleveland SC | Cleveland | 2018 | Don Shula Stadium |
| Colorado Crimson | — | 2007 | — |
| Dakota Fusion FC | Fargo | — | — |
| Denver Kickers | — | 1962 | — |
| Duluth FC | Duluth | 2015 | Denfeld High School |
| Eau Claire Aris FC | — | 2009 | — |
| El Farolito Soccer Club | San Francisco | 1985 | Boxer Stadium |
| Erie Admirals S.C. | Erie | 2009 | — |
| F.C. New York | Queens | 2009 | Belson Stadium |
| FC Arizona | Mesa | 2016 | — |
| FC Columbus | Columbus | — | — |
| FC Hasental | — | 2005 | — |
| FC Monmouth | Monmouth County | 2017 | — |
| FC Reading Revolution | — | 2009 | — |
| FC Santa Clarita | — | 2006 | — |
| FC Tacoma 253 | — | 2014 | — |
| FC Tulsa | — | 2009 | Memorial High School |
| FC Wichita | Wichita | 2013 | — |
| Galveston Pirate SC | — | 2010 | — |
| Georgia Revolution | McDonough | 2010 | Henry County High School |
| Gorge FC | — | 2014 | — |
| Grand Rapids Alliance | — | 2002 | — |
| Hartford City FC | Hartford | 2015 | — |
| Houston Hurricanes FC | — | December 2012 | Lutheran South Academy |
| Idaho Wolves | — | 2004 | — |
| Indianapolis Braves | — | 2007 | — |
| Inter Nashville FC | Nashville | — | — |
| Kalamazoo FC | Kalamazoo | 2015 | — |
| Kingston Stockade Football Club | Kingston | 2015 | Dietz Stadium |
| Knoxville Force | Knoxville | 2010 | — |
| Kraze United | — | 2015 | UCF Soccer and Track Complex |