| Austin Aztex | Austin | 2011 | House Park |
| Austin Bold FC | Austin | 9 August 2017 | Bold Stadium |
| Buffalo Pro Soccer | Buffalo | 2024 | Queen City Field |
| California Jaguars | — | 1995 | Salinas Sports Complex |
| Colorado Springs Switchbacks FC | Colorado Springs | 2013 | Switchbacks Training Stadium |
| Detroit City FC | Detroit | 2012 | Keyworth Stadium |
| El Paso Locomotive FC | El Paso | 1 March 2018 | New El Paso Ballpark |
| F.C. New York | Queens | 2009 | Belson Stadium |
| FC Tulsa | Tulsa | December 2013 | Oneok Field |
| Hartford Athletic | Hartford | 11 July 2018 | Trinity Health Stadium |
| Indy Eleven | Indianapolis | 2013 | IU Michael A. Carroll Track & Soccer Stadium |
| Las Vegas Lights FC | Las Vegas | 11 August 2017 | Cashman Field |
| Loudoun United FC | Leesburg | 18 July 2018 | Audi Field |
| Louisville City FC | Louisville | 4 June 2014 | Lynn Family Stadium |
| Memphis 901 FC | Memphis | 8 January 2018 | AutoZone Park |
| Miami FC | Miami | 2015 | Pitbull Stadium |
| Nashville SC | Nashville | 19 May 2016 | First Horizon Park |
| New Mexico United | Albuquerque | 6 June 2018 | Isotopes Park |
| North Carolina FC | Cary | 26 January 2006 | WakeMed Soccer Park |
| Oakland Roots SC | Oakland | 2018 | Oakland Coliseum |
| OKC Energy FC | Oklahoma City | 2 July 2013 | Taft Stadium |
| Orange County SC | Irvine | 2010 | Championship Soccer Stadium |
| Orlando City SC | — | 2010 | Camping World Stadium, ESPN Wide World of Sports Complex |
| Phoenix FC | Phoenix | 2 July 2012 | Sun Devil Soccer/Lacrosse Stadium |
| Phoenix Rising FC | Peoria | 13 March 2014 | Phoenix Rising Soccer Stadium, Peoria Sports Complex, Casino Arizona Field, Scottsdale Stadium |
| Pittsburgh Riverhounds SC | Pittsburgh | 11 March 1998 | Highmark Stadium |
| Puerto Rico United | Aguada | 2007 | Aguada Stadium |
| Queensboro FC | Queens | 12 November 2019 | — |
| Reno 1868 FC | Reno | 16 September 2015 | Greater Nevada Field |
| Rio Grande Valley FC Toros | Edinburg | 15 July 2015 | Robert and Janet Vackar Stadium |
| Sacramento Republic FC | Sacramento | 3 December 2012 | Heart Health Park |
| Saint Louis FC | St. Louis | 1 May 2014 | World Wide Technology Soccer Park |
| San Antonio FC | — | 7 January 2016 | Toyota Field |
| Sporting Club Jacksonville men | — | 30 August 2022 | — |
| USL Chicago | Chicago | 21 November 2017 | Lincoln Yards Stadium |
| VSI Tampa Bay FC | Plant City | 16 November 2011 | Plant City Stadium |
| Wilmington Hammerheads FC | Wilmington | 1996 | Legion Stadium |