| Club | Based in | Founded | Home ground |
|---|---|---|---|
| Atlanta United FC | Atlanta | 2014 | Mercedes-Benz Stadium |
| Austin FC | Austin | 2018 | Q2 Stadium |
| Charlotte FC | Charlotte | 17 December 2019 | Bank of America Stadium |
| Chicago Fire FC | Chicago | 8 October 1997 | Soldier Field |
| Chivas USA | Los Angeles | 2004 | Dignity Health Sports Park |
| Colorado Rapids | Commerce City | 1995 | Dick's Sporting Goods Park |
| D.C. United | Washington, D.C. | 1994 | Audi Field |
| FC Cincinnati | Cincinnati | 12 August 2015 | TQL Stadium |
| FC Dallas | Frisco | 1995 | Toyota Stadium |
| Houston Dynamo FC | Houston | 2005 | Shell Energy Stadium |
| Inter Miami CF | Miami | 29 January 2018 | Chase Stadium |
| LA Galaxy | Los Angeles | 1995 | Dignity Health Sports Park |
| Los Angeles FC | Los Angeles | 2014 | BMO Stadium |
| Miami Fusion | Fort Lauderdale | 1997 | Lockhart Stadium |
| Minnesota United FC | Minneapolis | 2015 | Allianz Field |
| Nashville SC | Nashville | 2017 | Nissan Stadium, Geodis Park |
| New England Revolution | Foxborough | 1995 | Gillette Stadium |
| New York City FC | New York City | 21 May 2013 | Yankee Stadium |
| Philadelphia Union | Chester | 2008 | Subaru Park |
| Portland Timbers | Portland | 2009 | Providence Park |
| Real Salt Lake | Salt Lake City | 2005 | America First Field |
| Red Bull New York | Harrison | 1995 | Giants Stadium, Sports Illustrated Stadium |
| San Diego FC | San Diego | 18 May 2023 | Snapdragon Stadium |
| Seattle Sounders FC | Seattle | 2009 | Lumen Field |
| St. Louis City SC | St. Louis | 20 August 2019 | Energizer Park |
| Tampa Bay Mutiny | — | 1995 | Raymond James Stadium |
| Toronto FC | Toronto | 2006 | BMO Field |