| Atlanta Silverbacks U23's | USL League Two | — | Atlanta Silverbacks Park |
| Bermuda Hogges | USL League Two | Pembroke Parish | Bermuda National Stadium |
| Boston Aztec | National Premier Soccer League | — | — |
| California Victory | USL First Division | San Francisco | Kezar Stadium |
| Chicago Stars FC | National Women's Soccer League | Chicago | SeatGeek Stadium |
| Chicago United Breeze | Women's Premier Soccer League | — | — |
| Claremont Stars | Women's Premier Soccer League | — | — |
| Cleveland City Stars | USL First Division | Cleveland | Middlefield Cheese Stadium |
| Crystal Palace Baltimore | USSF Division 2 Professional League | Baltimore | — |
| Delaware Dynasty | USL League Two | — | — |
| Detroit Ignition | — | Plymouth | USA Hockey Arena |
| FC Santa Clarita | National Premier Soccer League | — | — |
| Fort Lauderdale Strikers | North American Soccer League | Fort Lauderdale | Lockhart Stadium |
| Fredericksburg Gunners | USL League Two | — | Fredericksburg Academy |
| Gotham FC | National Women's Soccer League | Whippany | Sports Illustrated Stadium |
| Long Island Academy | National Premier Soccer League | — | — |
| Mississippi Brilla | USL League Two | Clinton | — |
| North Carolina FC | USL Championship | Cary | WakeMed Soccer Park |
| Ogden Outlaws | USL League Two | — | Bonneville High School |
| Phoenix Banat Storm | National Premier Soccer League | — | — |
| Queen City FC | National Premier Soccer League | — | All-High Stadium |
| Real Shore F.C. | National Premier Soccer League | — | — |
| Redwood City Ruckus | National Premier Soccer League | — | — |
| San Fernando Valley Quakes | USL League Two | Calabasas | — |
| Sevilla FC Puerto Rico | Liga Nacional de Fútbol de Puerto Rico | Juncos | Juan Ramón Loubriel Stadium |
| Sound FC | USL League Two | Sumner | — |
| Spokane Spiders | USL League Two | — | Joe Albi Stadium |
| Springfield Demize | USL League Two | Springfield | — |
| St. Louis Lions | USL League Two | St. Louis | — |
| Toronto FC | Major League Soccer | Toronto | BMO Field |
| Ventura County Fusion | USL League Two | Ventura | Ventura College |
| Virginia Beach Piranhas | USL League Two | — | Virginia Beach Sportsplex |