| Asheville City SC | National Premier Soccer League | Asheville | Memorial Stadium, Asheville |
| Ballista FC | Puerto Rico Soccer League | — | — |
| Caguas Sporting FC | Puerto Rico Soccer League | — | — |
| Cedar Rapids Rampage United | United Premier Soccer League | — | — |
| Charlottesville Alliance FC | National Premier Soccer League | Charlottesville | Charlottesville High School |
| El Paso Coyotes | Major Arena Soccer League | — | El Paso County Coliseum |
| FC Arizona | National Premier Soccer League | Mesa | — |
| Houston FC | United Premier Soccer League, USL League Two | Houston | — |
| Nashville SC | USL Championship | Nashville | First Horizon Park |
| Nashville SC U23 | USL League Two | — | FirstBank Stadium |
| Oxnard Guerreros FC | National Premier Soccer League | Temecula | — |
| Port City FC | National Premier Soccer League | — | — |
| San Antonio FC | USL Championship | — | Toyota Field |
| San Diego Zest FC | USL League Two | San Diego | RIMAC Sports Complex |
| San Francisco Deltas | North American Soccer League | San Francisco | Kezar Stadium |
| Shreveport Rafters FC | National Premier Soccer League | Shreveport | Independence Stadium |
| Sioux Falls Thunder FC | National Premier Soccer League | — | — |
| Toledo United FC | Great Lakes Premier League | — | Springfield Local High School |
| Treasure Coast Tritons | USL League Two | Port St. Lucie | — |
| Tyler FC | National Premier Soccer League | Tyler | Brook Hill School |
| Virginia United FC | — | Woodbridge | — |