| Club | Based in | Founded | Home ground |
|---|---|---|---|
| California United Strikers FC | Fullerton | 2017 | Titan Stadium |
| Chicago House AC | Chicago | 2020 | — |
| Gold Star FC Detroit | — | 2022 | — |
| Los Angeles Force | Los Angeles | 2019 | — |
| Maryland Bobcats FC | New York City | 2010 | Maryland SoccerPlex |
| Michigan Stars FC | Detroit | 1982 | Wisner Stadium |
| New Amsterdam FC | New York City | 2020 | — |
| New York Cosmos | New York City | 1 August 2010 | Maimonides Park |
| San Diego 1904 FC | San Diego | 2017 | Torero Stadium |
| Stumptown AC | Matthews | 2018 | Sportsplex at Matthews |