| Club | Based in | Founded | Home ground |
|---|---|---|---|
| FC Tucson | Arizona | 2010 | Veterans Memorial Stadium |
| Forward Madison FC | Madison | 17 May 2018 | Breese Stevens Field |
| Greenville Triumph SC | Greenville | 13 March 2018 | — |
| New York Cosmos | Paterson | 2025 | Hinchliffe Stadium |
| Northern Colorado Hailstorm FC | — | 12 January 2021 | — |
| One Knoxville SC | Knoxville | 2021 | — |
| Portland Hearts of Pine | — | 7 September 2023 | Fitzpatrick Stadium |
| Richmond Kickers | Richmond | 1993 | City Stadium |
| Sarasota Paradise | Lakewood Ranch | 19 November 2022 | — |
| Spokane Velocity | Spokane | 2021 | — |
| Tormenta FC | Statesboro | 2015 | Bo Pitts Field |
| Union Omaha | Omaha | 2019 | — |