| El Paso Patriots | El Paso | 1989 | Patriot Stadium |
| Evergreen FC | Leesburg | 2015 | — |
| FC Buffalo | Buffalo | 2009 | Buffalo State University |
| FC JAX Destroyers | — | 2010 | D. B. Milne Field |
| FC Miami City | Miami | 2014 | Tropical Park Stadium |
| Flint City Bucks | Flint | 1995 | Ultimate Soccer Arenas |
| Fort Lauderdale Schulz Academy | — | 2009 | — |
| Fredericksburg Gunners | — | 2006 | Fredericksburg Academy |
| Fredericksburg Hotspur | — | 2010 | — |
| Fresno Fuego | Fresno | 2003 | Chukchansi Park |
| GPS Portland Phoenix | Portland | 2009 | Memorial Stadium |
| Houston FC | Houston | 2016 | — |
| Houston Leones | — | 2007 | — |
| Houston Toros | — | 2002 | — |
| IMG Academy Bradenton | Bradenton | 1998 | — |
| Indiana Blast | — | 1997 | Kuntz Stadium |
| Indiana Invaders | — | 1998 | Indiana Invaders Soccer Complex |
| Jackson Chargers | Jackson | 1995 | — |
| Jersey Express S.C. | Newark | 2007 | Elizabeth High School |
| Jersey Shore Boca | — | 2003 | Ocean County College |
| Kalamazoo Kingdom | — | 1996 | — |
| Kalamazoo Outrage | — | 2007 | Loy Norrix High School |
| Kitsap Pumas | Bremerton | 2009 | — |
| Lafayette Swamp Cats | — | 2000 | — |
| Laredo Heat | Laredo | 2004 | Texas A&M International University Soccer Complex |
| Long Island Rough Riders | Long Island | 1994 | Hofstra University Soccer Stadium |
| Los Angeles Heroes | — | 1993 | — |
| Memphis Express | — | 2002 | — |
| Mississippi Brilla | Clinton | 2006 | — |
| Montgomery United FC | Montgomery | 2024 | — |
| Nashville Metros | Nashville | 1989 | E. S. Rose Park |
| Nashville SC U23 | — | 2016 | FirstBank Stadium |
| Nevada Wonders | — | 2003 | Carson High School |
| New Jersey Stallions | — | 1996 | DePaul Catholic High School |
| North Jersey Imperials | — | 1994 | — |
| Northern Arizona Prospectors | Arizona | 1998 | Bradshaw Mountain High School |
| Northern Virginia FC | — | 1998 | — |
| OC Blues Strikers FC | — | 2011 | — |
| Ocean City Nor'easters | Ocean City | 1996 | Carey Stadium |
| Ogden Outlaws | — | 2006 | Bonneville High School |
| Orange County Blue Star | Irvine | 1997 | — |
| Panama City Beach Pirates | Panama City Beach | 2007 | Arnold High School |
| Park City Red Wolves SC | Park City | — | — |
| Portland Timbers U23s | — | 2008 | Providence Park |
| Puget Sound Gunners FC | — | 2010 | Issaquah High School |
| Reading United AC | Reading | 1996 | — |
| Real Boston Rams | — | 2012 | Veterans Memorial Stadium |
| Real Colorado SC | — | 1986 | — |