| Cape Cod Crusaders | USL League Two | Massachusetts | Massachusetts Maritime Academy |
| Cincinnati Cheetahs | United Soccer League, USL Second Division | — | RDI Stadium |
| Colorado Pride | — | — | Washburn Field |
| D.C. United | Major League Soccer | Washington, D.C. | Audi Field |
| Des Moines Menace | USL League Two | Des Moines | Valley High School |
| Houston Hotshots | Continental Indoor Soccer League | — | Lakewood Church Central Campus |
| Long Island Rough Riders | A-League, USL Second Division, USL League Two | Long Island | Hofstra University Soccer Stadium |
| Long Island Rough Riders women | United Women's Soccer | Uniondale | Hofstra University Soccer Stadium |
| New York Centaurs | A-League | Randalls Island | Downing Stadium |
| North Jersey Imperials | USL First Division, USL Second Division, USL League Two | — | — |
| Pittsburgh Stingers | Continental Indoor Soccer League | — | Civic Arena |
| Seattle Sounders | USL First Division, A-League | — | Starfire Sports |
| St. Louis Knights | — | St. Louis | — |
| Tornados de Humacao | Puerto Rico Soccer League | Humacao | Nestor Morales Stadium |
| Virginia Beach Mariners | USL First Division | Virginia Beach | Virginia Beach Sportsplex |